About Pimm and author

About pimm

Partial immortalization – Pimm is an online bookblog about maximum life extension and regenerative medicine. Please participate and comment because the true value and quality of the adventure needs your contribution, which I would really appreciate. Thanks, Attila.

About author

Attila Chordash (say tshor-dash) = Attila Csordás (Hungarian)

I am a last year PhD student, a trained molecular biologist and biotechnologist, and my topic is stem cell biology, tissue engineering, regenerative medicine and mitochondrial biology. I got a master's degree in philosophy too.

Research Interests

Stem cell biology and therapy, tissue engineering, regenerative medicine, mitochondrial bioenergetics, the role of mitochondria in aging, bioinformatics, systems biology, biological databases, bioethics

Research Techniques/Computer Techniques

• In vitro cell culture techniques • Isolation of human cardiomyocytes, human amniotic and human mesenchymal stem cells • Stem cell culture maintenance: human amniotic cells, human and mouse mesenchymal cells • Laser scanning confocal microscopy • Polymerase Chain Reaction: mitochondrial DNA • Single-stranded Conformation Polymorphism (SSCP) • Mitochondria isolation • Immunocytochemical staining • Flow cytometry • Transplantation techniques on rats: local and systematic • Cold lesion model on rat and mouse • Rat neurological test • Optical Waveguide Lightmode Spectroscopy (OWLS) • Planning and building biological databases

Publications/Presentations

Csordás A, Pankotai E, Snipes JA, Sárszegi Z, Cziráki A, Gaszner B, Papp L, Benkő R, Kiss L, Kovács E, Kollai M, Szabó C, Busija DW, Lacza Z
Human heart mitochondria do not produce physiologically relevant quantities of nitric oxide
(submitted for publication in Life Sciences)

Lacza Z, Kozlov AV, Pankotai E, Csordas A, Wolf G, Redl H, Kollai M, Szabo C, Busija DW, Horn TF. Mitochondria produce reactive nitrogen species via an arginine-independent pathway. Free Radic Res. 2006 Apr;40(4):369-78.

Lacza Z, Pankotai E, Csordas A, Gero D, Kiss L, Horvath EM, Kollai M, Busija DW, Szabó C. Mitochondrial NO and Reactive Nitrogen Species Production: Does mtNOS exist?
Nitric Oxide. 2006 Mar;14(2):162-8.

Horváth EM, Lacza Z, Csordás A, Szabó C, Kollai M, Busija DW. Graft derived cells with double nuclei in the penumbral region of experimental brain trauma.
Neurosci Lett. 2005 Dec 22;

Lacza Z, Horváth EM, Pankotai E, Csordás A, Kollai M, Szabó C, Busija DW. The novel red-fluorescent probe DAR-4M measures reactive nitrogen species rather than NO.
J Pharmacol Toxicol Methods. 2005 Nov-Dec;52(3):335-40.

Lacza Z, Horváth EM, Csordás A, Kollai M, Szabó C and Busija DW Proceedings abstract on Transplanted stem cells undergo cellular fusion in experimental brain trauma, Journal of Cerebral Blood Flow & Metabolism (2005) 25, S517. Gene Expression and Cell Therapy Poster Session

Csordás A – Lacza Zs: Stem cells and regenerative medicine, (in Hungarian), Praxis, 2005. June, year 14/ issue 6

Csordás A., Pankotai E., Sárszegi Zs., Cziráki A., Kollai M., Szabó Cs., Lacza Zs.: Poster on Lack of Mitochondrial NO Synthase System in Human Heart, General meeting of Hungarian Physiological Society (MÉT), June 2005

Csordás A., Pankotai E., Sárszegi Zs., Cziráki A., Kollai M., Szabó Cs., Lacza Zs.: Presentation (in Hungarian): Human heart mitochondria do not produce NO, Cardiological Conference, May 2005

Csordás A., Pankotai E., Sárszegi Zs., Cziráki A., Kollai M., Szabó Cs., Lacza Zs., Poster on Human heart mitochondria do not produce NO, Semmelweis Medical University, PhD Days, April 15, 2005

Education

2003- Ph.D. student, completing all coursework (all credit), at the Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary topic: the role of mitochondria in ischaemic and degenerative diseases, stem cell transplantation, regenerative medicine

2003 August-2003 February assistant researcher at the Institute of Experimental Medicine, Laboratory of Neural and Cell Biology, Budapest, Hungary 2005-1999 Master of Philosophy at the Eötvös Loránd University (ELTE), Budapest, Hungary; Thesis: The Philosophical Problems of Biotechnology Supervisor: professor György Bence

1999-1993 MSc Biology at the Szeged University (JATE), Szeged, Hungary; Thesis: Mitochondrial Mutations and Human Ageing. Supervisor: Dr. Pál Venetianer

1999-1996 research on mitochondrial mutations and human ageing at the Scientific Students' Associations of the Hungarian Academy of Sciences, Biological Research Centre, Szeged, Hungary

Background/Additional Information Technology and science journalist for general audience: infotech and biotech, papers in philosophy in English and Hungarian.
Running a Go board game club

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